Fig. 2
From: Ultra-precise detection of mutations by droplet-based amplification of circularized DNA
The depth distribution of Droplet-CirSeq and Cir-seq libraries. The first and last 100 bp of the genomes were excluded. a Boxplot of depth distribution; y axis represents the log(e) ratio of depth over mean of E. coli genome. Two repeats of two different types of libraries were plotted. The Droplet-CirSeq libraries (Droplet-3 pg-1 and Droplet-3 pg-2) showed more concentration depth distribution and more closeness to the mean value than the Cir-seq libraries (Cir-3 pg-1 and Cir-3 pg-2). b The phix174 depth distribution of the Cir-seq (90 bp fragments) and standard NGS libraries (300 bp, 90 bp fragments). c The depth CV (coefficient of variance) of the Droplet-CirSeq, Cir-seq, and standard NGS libraries. The Droplet-CirSeq library had a depth CV of almost 10 times less than that of the Cir-seq library (p = 2.6 X 10-3), but it was still higher than that of the standard NGS library. d The depth LOWESS (locally weighted scatter plot smoothing) of the entire E. coli genome (10 bp slide window size). The red and yellow lines represent the LOWESS of Droplet-CirSeq, while the blue and cyan lines represent the LOWESS of Cir-seq