Fig. 3
From: Ultra-precise detection of mutations by droplet-based amplification of circularized DNA
Error rate and mutation types of Droplet-CirSeq. a Error rate of Droplet-CirSeq and Cir-seq. The “1X allele” represents the bases that were supported at least by one circularized DNA, while the “2X allele” represents the bases that were supported by at least two different circularized DNAs. The error rate of Droplet-CirSeq was 5.23 X 10-5 (±1.54 X 10-5) at the “1X allele” criterion and 3.71 X 10-6 (±2.34 X 10-7) at the “2X allele” criterion. b Mutation types of Droplet-CirSeq. The error rates of most of the mutation types were lower than 3.00 X 10-6, but the error rates for the transitions C= > T and G= > A were almost one order of magnitude higher than the other types, and the other two transitions, A= > G and T= > C, also showed high error rates. The mutation pattern of the “2X allele” showed the same pattern as with the “1X allele”