Experimental strategy. a Summary: we FACS sort embryonic cells, based on expression of markers with known expression patterns, and measure expression in cells expressing (or not expressing) a particular marker using RNA-seq. Genes expressed in similar sets of cells are enriched in a similar set of samples. b Expression patterns of cells used for sorting (shown in red), and in Spencer et al. , shown in yellow. Cell fates are shown in the colored bar at the top. c Expression pattern of unc-130 (one of the markers used for sorting) in the Abpl sublineage, with cell fates colored as in (b). d Comparison of overlap of groups of cells used for sorting in this paper, with similar overlap for the groups of cells used in Spencer et al.