Fig. 1From: Targeted alignment and end repair elimination increase alignment and methylation measure accuracy for reduced representation bisulfite sequencing dataRRBS concept and workflow. Genomic DNA is first digested by MSP1 which cuts at CCGG sites. These fragments are end-repaired, A-tailed, and adapter-attached and then bisulfite treated. The unmethylated C is converted to U (T) and methylated C is unchanged. The blue bases are from media and are not real biological signal that needed to be removedBack to article page