The major histocompatibility complex in Old World camelids and low polymorphism of its class II genes

Plasil, Martin; Mohandesan, Elmira; Fitak, Robert R.; Musilova, Petra; Kubickova, Svatava; Burger, Pamela A.; Horin, Petr

doi:10.1186/s12864-016-2500-1

BMC Genomics

Table 3 Primers used to amplify different MHC sequences in Old World camelids

From: The major histocompatibility complex in Old World camelids and low polymorphism of its class II genes

Locus	Name	Sequence (5' → 3')	Purpose	PCR product length
DRA	DRA-EXON2-F	TGAGAATTTTGGGTTTGCTTATGGCA	Bact, drom	514 bp
	DRA-EXON2-R	CCTCTGAGCAACACGAACGTCCTTCA	Bact, drom	514 bp
	DRA-EXON2-WC-F	TGGGTGTTTCAGCTCTTGTG	Ferus	650 bp
	DRA-EXON2-WC-R	AGATACCATGGGTGGCAAAG	Ferus	650 bp
	DRA-EXON2-3-F	CCCTGGAATTCGGGTTTAAG	Preventing allele dropout	923 bp
	DRA-EXON2-3-R	GGCTGAAAAAGCAGTTGAGC	Preventing allele dropout	923 bp
	DRA_F1	TGATCATCCAGGCTGAGTTC	Ancient samples	139 bp
	DRA_R1	GCAAACCGTCCAAATTCTTC	Ancient samples	139 bp
	DRA_F2	CGTGGATCTGGAAAAGAAGG	Ancient samples	154 bp
	DRA_R2	ATTGGTGTTCGGGGTGTG	Ancient samples	154 bp
DRB	DRB-EXON2-2-F	AGCAGTGGGGGTCCTAGTG	Bact, drom	457 bp
	DRB-EXON2-2-R	ACCCACCCGGACTCAGTATC	Bact, drom	457 bp
	DRB-EXON2-WC-F	TTCAGGAGGAGGTGGTGATG	Ferus	764 bp
	DRB-EXON2-WC-R	CTCAGACCCCAGACCCATT	Ferus	764 bp
	DRB-EXON2-3-F	GCCAGCCCTAGGCAAGTAAG	Preventing allele dropout	852 bp
	DRB-EXON2-3-R	GTTCTCTCAGACCCCAGACC	Preventing allele dropout	852 bp
DQA	DQA1-EXON2-F	CATGAAAGTCAATTTATCCTGTCAC	Bact	351 bp
	DQA-EXON2-2-R	AGTGAGGCCTGGTATGAAGG	Bact	351 bp
	DQA-EXON2-WC-F	ACGTGCTGGGAATTTTGTCT	Drom	414 bp
	DQA-EXON2-2-R	AGTGAGGCCTGGTATGAAGG	Drom	414 bp
	DQA-EXON2-WC-F	ACGTGCTGGGAATTTTGTCT	Ferus	646 bp
	DQA-EXON2-WC-R	GCTATGGGAGCTTTCCTTGA	Ferus	646 bp
	DQA-EXON2-4-F	ATGGTGCAGAGAGCAGAAGG	Preventing allele dropout	1096 bp
	DQA-EXON2-4-R	TGGGAAACACAGTCACCTCA	Preventing allele dropout	1096 bp
	DQA_F1	YGGCRTAAATGTCTACCAGTC	Ancient samples	133 bp
	DQA_R1	TSRAAACTTGSTAAACAGAGG	Ancient samples	133 bp
	DQA_F2	CGTGGACTTGGAGAAGAAGG	Ancient samples	145 bp
	DQA_R2	AGCAGTAGAGTTGGAGCGTTT	Ancient samples	145 bp
DQB	GH 28	CTCGGATCCGCATGTGCTACTTCACCAACG	Bact, drom, ferus	181 bp
DQB	GH 29	GAGCTGCAGGTAGTTGTGTCTGCACAC	Bact, drom, ferus	181 bp
FANCA	KN276514-F	TCAAGTCAGAAACAAGAACACAGA	FISH probe MHCII	3574 bp
FANCA	KN276514-R	CATCATTGCCAGATCCTTCA	FISH probe MHCII	3574 bp
TRIM39	KN277189-F	CCACCCACCATGTGTATGAA	FISH probe MHCI	3459 bp
TRIM39	KN277189-R	GCTGAGAGTGCAGGGATAGG	FISH probe MHCI	3459 bp

Bact - Primer pair used to amplify samples of Camelus bactrianus. Drom - Primer pair used to amplify samples of Camelus dromedarius. Ferus - Primer pair used to amplify samples of Camelus ferus

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ISSN: 1471-2164

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