Fig. 6

Direct sequencing of bisulfite PCR products of Stan (GB18207) from colony 2 and 3 fertilized and thelytokous embryos. a Summary of the 794 bp region of Stan used for bisulfite PCR sequencing. The location of Stan exons are indicated in orange. Methylated sites determined in the whole genome bisulfite sequencing of colony 1 are shown for fertilized (purple) and thelytokous (green). The * indicates CG sites that were deemed as hypermethylated in fertilized embryos, where 6 of the 7 hypermethylated sites are encompassed by the bisulfite PCR region. b&c Allele-specific methylation patterns of colony 2 (b) and colony 3 (c) fertilized and thelytokous embryos. The total number of CG sites present in the bisulfite PCR region is indicated as open ovals below exons (orange). Methylated CG sites are indicated as red ovals, and SNPs are designated as blue rectangles. The 3 alleles are labeled P1 (allele of paternal origin) and M2 and M3 (alleles of maternal origin)