|
Name
|
miRNA _sequence
|
Length
|
Contig
|
miRNA* sequence
|
Pipeline usedb
|
|---|
|
miRn1
|
TGCGTTTGCACCTGCACCTCT
|
21
|
scaffold303.0
|
AAGGTGCAGGTGTAACTGCAG
|
miRCAT
|
|
miRn2
|
CGAAGACCTTGGGGAGAGTGG
|
21
|
scaffold1160.0
|
ACTCTCCCTCAAGGGCTTCGA
|
miRCAT
|
|
miRn3
|
AAGCGGAGTAATGGTTACTGA
|
21
|
scaffold276.0
|
AGAGCTGCATTATTCTCTTGT
|
ShortStack
|
|
miRn4
|
TCACCTTGTAAAAAATTGGCC
|
21
|
scaffold266.0
|
CAATTTTTTATAAGGTGAAT
|
ShortStack
|
|
miRn5
|
TCGGGACAGCGTACTTGAGTT
|
21
|
scaffold230.0
|
CTCACGCGCGCTGTCCCGAGA
|
ShortStack
|
|
miRn6
|
TTGAGGATGCATAGTTTTCAG
|
21
|
scaffold266.0
|
GAAAATAATGCATCTTCAACG
|
ShortStack, miRCAT
|
|
miRn7
|
GTGTAACCGTCGTAATGTCCC
|
21
|
scaffold198.0
|
GACATTACAACGGCCACACGG
|
ShortStack
|
|
miRn8
|
CTGCCAAAGGAGATCTGCTCAG
|
22
|
scaffold1.0
|
GAGCAGTCTCCTCTTGGCAAAC
|
ShortStack,miRCAT
|
|
miRn9
|
TCCCTAAAACCACCAAGGCCAA
|
22
|
scaffold2.0
|
GGCTTTAGTGGGTTAGGAAGA
|
ShortStack
|
|
miRn10
|
GCGTACGAGGAGCCAAGCATA
|
21
|
scaffold32.0
|
TGCCTGGCTCCCTGTATGCCA
|
miRCAT
|
|
miRn11
|
AATAATTGTAGTCGTTGGATCA
|
22
|
scaffold371.0
|
TTTGATCTAACGGCTACAAACA
|
miRCAT
|
|
miRn12
|
TGGTGTTTGGATGGACGTGTT
|
21
|
scaffold862.0
|
TAAGTTCATCCAAACACCATA
|
ShortStack
|
|
miRn13
|
AGGTGCAGGTGTAACTGCAGA
|
21
|
scaffold303.0
|
TGCGTTTGCACCTGCACCTCT
|
ShortStack
|
|
miRn14
|
ACCTAGCTCTGATACCATAAA
|
21
|
scaffold3.0
|
TGTGGTATCAGGACTATGTTA
|
ShortStack
|
|
miRn15
|
GGTTTGAGTGGATTGGGAAGA
|
21
|
scaffold2.0
|
TTCTTAAAACCACTCAAGCCAA
|
ShortStack, miRCAT
|
|
miRn16
|
TTCTTGACCACCGACGCCGACA
|
22
|
scaffold131.0
|
TTGGCGTCGTGGTCAAGAAGG
|
ShortStack
|
|
miRn17
|
AAAACCGCGGATTGGGGCGTG
|
21
|
scaffold17.0
|
NO
|
miRCAT
|
|
miRn18
|
AAGAGATATGGACCGTTGGATA
|
22
|
scaffold1.0
|
NO
|
miRCAT
|
|
miRn19
|
AATGACGTGTGGCATATCATC
|
21
|
scaffold28.0
|
NO
|
miRCAT
|
|
miRn20
|
ACACGATGTATGATGAACGG
|
20
|
scaffold1044.0
|
NO
|
miRCAT
|
|
miRn21
|
ACCTGATTGGTTGCTGTTGGAT
|
22
|
scaffold89.0
|
NO
|
miRCAT
|
|
miRn22
|
ACTTTGGGATGTGGCAATGTGA
|
22
|
scaffold285.0
|
NO
|
miRCAT
|
|
miRn23
|
AGGAGACGAAGAAACTGGTGC
|
21
|
scaffold463.0
|
NO
|
miRCAT
|
|
miRn24
|
AGGAGAGGGAGGTGGGTCGGG
|
21
|
scaffold6.0
|
NO
|
miRCAT
|
|
miRn25
|
AGGATGAGCTGAAGATGATAA
|
21
|
scaffold1.0
|
NO
|
miRCAT
|
|
miRn26
|
CAAAGTTTTTGGAATGTTGCA
|
21
|
scaffold54.2
|
NO
|
miRCAT
|
|
miRn27
|
CCGAACTTGGTGGATTAGGAG
|
21
|
scaffold1.0
|
NO
|
miRCAT
|
|
miRn28
|
CCGGATTTCGTGGTCAGGAGG
|
21
|
scaffold124.0
|
NO
|
miRCAT
|
|
miRn29
|
CCTGACTGTTGATGCATGTAGG
|
22
|
scaffold373.0
|
NO
|
miRCAT
|
|
miRn30
|
CCTTGTTTAGGGTATGTAGGCA
|
22
|
scaffold1113.0
|
NO
|
miRCAT
|
|
miRn31
|
CGAACTTGGTGGATTAGGAGG
|
21
|
scaffold30.0
|
NO
|
miRCAT
|
|
miRn32
|
CGAGGGTGTGGTGTAGGGTGG
|
21
|
scaffold181.0
|
NO
|
miRCAT
|
|
miRn33
|
CGATGAACGGACGTGATTGGA
|
21
|
scaffold10.0
|
NO
|
miRCAT
|
|
miRn34
|
CGATGTACGATGAACGGACACA
|
22
|
scaffold1279.0
|
NO
|
miRCAT
|
|
miRn35
|
CGCCTTGGCGAAACTCTAGGAA
|
22
|
scaffold310.0
|
NO
|
miRCAT
|
|
miRn36
|
CGGAATACGGATGGTACACCA
|
21
|
scaffold63.0
|
NO
|
miRCAT
|
|
miRn37
|
CGGATTTCGTGGTCAGGAGGA
|
21
|
scaffold228.0
|
NO
|
miRCAT
|
|
miRn38
|
CGGTCAGTAGGATCCCAAGGCA
|
22
|
scaffold242.0
|
NO
|
miRCAT
|
|
miRn39
|
CGTACGATATACAATGAACGGT
|
22
|
scaffold171.0
|
NO
|
miRCAT
|
|
miRn40
|
CGTACGATCTACGATGAACGG
|
21
|
scaffold182.0
|
NO
|
miRCAT
|
|
miRn41
|
CTAATGACATTATAGAGGACA
|
21
|
scaffold103.0
|
NO
|
miRCAT
|
|
miRn42
|
CTGCACAATGTACGATGAACGG
|
22
|
scaffold13.0
|
NO
|
miRCAT
|
|
miRn43
|
CTGTACGATATATGATGAACGG
|
22
|
scaffold1196.0
|
NO
|
miRCAT
|
|
miRn44
|
GAATACGGATGGTACACCAT
|
20
|
scaffold330.0
|
NO
|
miRCAT
|
|
miRn45
|
GCATTTGGATTTGTCTGACTTG
|
22
|
scaffold1206.0
|
NO
|
miRCAT
|
|
miRn46
|
GGGACTTTCAAATTCCGAGGG
|
21
|
scaffold353.0
|
NO
|
miRCAT
|
|
miRn47
|
GTCAAACTGTGATTTGTAGGCA
|
22
|
scaffold94.0
|
NO
|
miRCAT
|
|
miRn48
|
GTGACTGATGACATGTTGTAG
|
21
|
scaffold322.0
|
NO
|
miRCAT
|
|
miRn49
|
TAGGCGTAGATAGACCGTGGG
|
21
|
scaffold144.0
|
NO
|
miRCAT
|
|
miRn50
|
TCAAATTTCAAAGGTCCGGATC
|
22
|
scaffold221.0
|
NO
|
miRCAT
|
|
miRn51
|
TCACACTATGGAGCGATGGTC
|
21
|
scaffold1.0
|
NO
|
miRCAT
|
|
miRn52
|
TCCTCGAACTGTAGCAATGGC
|
21
|
scaffold44.1
|
NO
|
miRCAT
|
|
miRn53
|
TCTAACGGTCAAGAGGATGTC
|
21
|
scaffold751.0
|
NO
|
miRCAT
|
|
miRn54
|
TCTGGATGCATGAATTTGGTA
|
21
|
scaffold83.0
|
NO
|
miRCAT
|
|
miRn55
|
TGAACGGACATGATTTGAGGA
|
21
|
scaffold3.0
|
NO
|
miRCAT
|
|
miRn56
|
TGGATTTGGTAGAAGGGATCC
|
21
|
scaffold12.0
|
NO
|
miRCAT
|
|
miRn57
|
TGTGATGTGTGGTTACGGTT
|
20
|
scaffold235.0
|
NO
|
miRCAT
|
|
miRn58
|
TTCAGGGCTCTGAGTGGGATGG
|
22
|
scaffold48.0
|
NO
|
miRCAT
|
|
miRn59
|
TTGGATTAAAATTGAACGGCC
|
21
|
scaffold114.0
|
NO
|
miRCAT
|
-
aAdditional file 4: Figure S1 showed the stem-loop structure of the predicted precursors of each pear-specific miRNAs
-
bPipeline(s) that successfully identified the miRNA loci
