Fig. 4

Predicted gene models of the C. metallifer transformer-2 (Cmtra2) gene. Nine different splice variants were predicted from the database assembly and annotation. A total of five different isoforms were predicted (isoform A-E). The coding sequences are in gray, stop codons are indicated by black arrowheads. The open arrowhead indicates the start codon. Arrows indicate forward and reverse primer locations for RT-PCR and the red bar indicates the region for dsRNA synthesis. The blue dashed box indicates the sequences which were identified by 5’ RACE