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Table 2 Summary of database search for candidate genes

From: Identification and functional analyses of sex determination genes in the sexually dimorphic stag beetle Cyclommatus metallifer

Candidate gene name (ab)

OrthoDB7_ID

Accession No. of Drosophila homolog for query

Accession No. of Tribolium homolog for query

Isotig number of the homolog or highest similarity (Drosophila query)

Isotig number of the homolog or highest similarity (Tribolium query)

e-value (Drosophila query)

e-value (Tribolium query)

Longest hit length and the percentage of amino acid identity (Drosophila query)

Longest hit length and the percentage of amino acid identity (Tribolium query)

Identified as Cyclommatus homolog of

groucho (gro)

EOG7BD0RV

NM_001260380

XM_963022

7235

7235

0

0

321/355 (90 %)

323/348 (93 %)

groucho (gro)

ovo/shavenbaby (ovo)

EOG7C0766

NM_001169202

XM_969788

7894

7894

9.00E-99

5.00E-131

133/157 (85 %)

181/192 (94 %)

ovo/shavenbaby (ovo)

sansfille (snf)

EOG78WZ6X

NM_078490

XM_963178

10553

10553

5.00E-88

2.00E-107

85/98 (87 %)

99/106 (93 %)

sansfille (snf)

hopscotch (hop)

EOG74JNNN

NM_078564

XM_969329

5920

5920

3.00E-87

0

39/69 (57 %)

194/248 (78 %)

hopscotch (hop)

fruitless (fru)

EOG7S84VN

NM_079673

NM_001164218

8868

8868

1.00E-76

3.00E-138

97/125 (78 %)

124/156 (79 %)

fruitless (fru)

virillizer (vir)

EOG7DRWGS

NM_080161

XM_001807225

8354

7116

9.00E-54

2.00E-179

62/80 (78 %)

81/126 (64 %)

virillizer (vir)

daughterless (da)

EOG7BGW18

NM_001273411

XM_968179

12902

12902

4.00E-53

4.00E-93

89/98 (91 %)

122/126 (97 %)

daughterless (da)

female lethal d (fl(2)d)

EOG7KT8QP

NM_166010

XM_969964

4453

4453

1.00E-51

2.00E-116

55/63 (87 %)

116/142 (82 %)

female lethal d (fl(2)d)

deadpan (dpn)

EOG7X6ZFD

NM_057575

XM_962601

10698

10698

7.00E-47

1.00E-80

73/118 (62 %)

109/132 (83 %)

deadpan (dpn)

transformer2 (tra2)

EOG7Z3SMD

NM_057416

XM_963457

1275

1275

2.00E-41

2.00E-79

59/107 (55 %)

111/120 (93 %)

transformer2 (tra2)

intersex (ix)

EOG7162KM

NM_136833

XM_008200746

10843

10843

4.00E-32

4.00E-68

29/47 (62 %)

108/143 (76 %)

intersex (ix)

Sex-lethal (Sxl)

EOG72ZQVX

NM_001031891

NM_001145943

4016

10319

4.00E-48

3.00E-42

63/128 (49 %)

68/100 (68 %)

Sex-lethal (Sxl)

extramacrochaetae (emc)

EOG7QCMBK

NM_079152

XM_964922

9148

9148

2.00E-26

1.00E-63

40/70 (57 %)

88/112 (79 %)

extramacrochaetae (emc)

dissatisfaction (dsf)

EOG7VJ5CF

NM_001273180

XM_970162

6548

6548

3.00E-43

3.00E-51

38/107 (36 %)

41/107 (38 %)

ultraspiracle (usp)

hermaphrodite (her)

EOG7JXFJ7

NM_001273577

Not found in OrthoDB

9417

NS

4.00E-10

NS

11/34 (32 %)

NS

Hypothetical protein

degringolade (dgrn)

EOG7KX50F

NM_141339

Not found in OrthoDB

7560

NS

2.00E-04

NS

12/27 (44 %)

NS

E3 ubiquitin-protein ligase

scute/sisterlessB (sc)

EOG7HJ870

NM_057455

Not found in OrthoDB

9495

NS

0.01

NS

14/24 (58 %)

NS

delilah (dei)

ovarian tumor (otu)

EOG7N9B8P

NM_001272403

EEZ98091

1660

1444

0.02

0.65

16/46 (35 %)

11/37 (30 %)

Hypothetical protein/terribly reduced optic lobes (trol)

standstill (stil)

EOG7RVNX6

NM_057404

Not found in OrthoDB

3203

NS

0.06

NS

11/34 (32 %)

NS

Brain-enriched guanylate kinase-associated protein

transformer (tra)

EOG7HN4HG

NM_079390

JQ857102

2890

4317

0.12

2.00E-08

10/27 (37 %)

17/41 (41 %)

mitochondrial ribosomal protein L1/ transformer (tra)

runt (run)

EOG73RPRJ

NM_078700

XM_964184

7872

5910

0.28

0.26

14/33 (42 %)

11/18 (61 %)

ribosomal protein L4e/egalitarian (egl)

doublesex (dsx)

EOG77DWNS

NM_169202

XM_001807396

2520

6093

0.55

0

10/19 (53 %)

16/31 (52 %)

NSFL1 cofactor p47/Ubiquinol-cytochrome c reductase core protein 1

sisterless-A (sisA)

EOG7FZBDN

NM_078561

Not found in OrthoDB

6705

NS

0.66

NS

17/45 (38 %)

NS

glypican-4

outstreched (os)

EOG7TJFZ9

NM_001103545

Not found in OrthoDB

3953

NS

0.85

NS

11/30 (37 %)

NS

High mobility group protein DSP1

  1. NS not searched
  2. Bold numbers indicated isotig numbers annotated as focal gene
  3. Genes indicated in bold denote the candidate genes identified and annotated in our C. metalifer transcriptome database