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Fig. 6 | BMC Genomics

Fig. 6

From: Reconstruction of gene regulatory modules from RNA silencing of IFN-α modulators: experimental set-up and inference method

Fig. 6

Validation of STAT1 negative regulation on IFNAR1. a Flow cytometric analysis of HUVEC cells transduced with a lentiviral vector coding wild-type STAT1 cDNA. STAT1 overexpression caused down-regulation of surface IFNAR1 expression after 16 h (D59 hTERT) and 24 h (both donors) of IFN-α stimulation in two different endothelial cell cultures (HUVEC D48 and D59 hTERT). Percentage of positive cells is reported for both HUVECs transduced with pLV-STAT1 and pLV-CTRL. IFNAR1 expression was evaluated relative to isotype control (not shown). b Western blot analysis of STAT1 levels in HUVECs used in (a). α-tubulin hybridization is shown as a loading control. For quantification of STAT1 expression, density values were normalized to the STAT1/α-tubulin ratio measured in the control sample (pLV-CTRL)

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BMC Genomics

ISSN: 1471-2164

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