Fig. 6

Results of RT-qPCR validation of high-throughput analyses data. a Comparative fold-changes (mean ± SEM) in miRNA abundance between pregnant and non-pregnant groups obtained by high-throughput analyses (PCR array or sequencing) and RT-qPCR. Results to the left and right of the dotted line correspond to NP16 vs P16 and NP vs P24 comparisons, respectively (Additional file 4). b RT-qPCR data plots (with mean ± SEM) for selected comparisons between pregnant and non-pregnant groups including the two differences in miRNA abundance that were significant (miR-26a, * indicates P < 0.05) or tended to be significant (miR-1249, NP vs P16, P < 0.1). c RT-qPCR data plots (with mean ± SEM) obtained from an independent group of heifers and which confirms an increase in plasma miR-26a levels during early pregnancy. NP corresponds to plasma samples collected before insemination (Day 0) from the same animals