Fig. 4From: RNA sequencing to characterize transcriptional changes of sexual maturation and mating in the female oriental fruit fly Bactrocera dorsalis Differentially expressed genes between mature virgin females and immature individuals in various tissues. qRT-PCR was used to analyze mRNA levels of differentially expressed genes, including those found in the head, thorax, ovary, fat body and midgut. Relative transcript levels are calculated by real-time PCR using the 16 s rRNA gene as the standard control. Various letters indicate significant differences in the expression level (p < 0.05, Duncan’s test). Three biological replicates were performedBack to article page