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Fig. 3 | BMC Genomics

Fig. 3

From: Defects in the NC2 repressor affect both canonical and non-coding RNA polymerase II transcription initiation in yeast

Fig. 3

Selected examples of verified ORF-genes showing cryptic transcription in their promoters. Screenshots from the tiling analysis software (TAS) are shown as example of the different cryptic transcripts detected after shutting down either BUR6 or YDR1. Intensity profiles on an arbitrary, but identical, scale for the mutants and wild type are shown. In (a, b and d), only the forward signal is shown, and both the forward and reverse intensity signals from the strand-specific microarray are shown in c. The SGD map for canonical genes is represented below and indicates the sense of transcription with an arrowhead. a) Cryptic transcription in the 5′ flank of the YOL039w/RRP2A gene in both NC2 mutants. b) Cryptic transcription in the 3′ flank of the YHR163w/SOL3 gene in both NC2 mutants. c) Cryptic transcription in the 5′ flank of the YDL133w/SRF1 gene in both NC2 mutants in both forward and reverse (antisense). d) Cryptic transcription in the 5′ flank of the YDR510w/SMT3 gene in both NC2 mutants. In this case the cryptic transcript could be detected with a probe from the YDR509w dubious ORF as in the macroarray experiment described in Fig. 1 and in the text

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