Table 3 Up-regulation of the R. tropici CIAT 899 pRtCIAT899b genes located downstream nod boxes. Transcriptional activation (4-fold induction with respect to control cultures) of several nod box controlled operons was demonstrated by RNA-seq data in the presence of both inducer molecules. HP: gene that codes for a hypothetical protein
NB (Distance) | Cds number/Locus tag/Gene name | Putative function | Fold change apigenin | Fold change salt |
|---|---|---|---|---|
1 (270 pb) | cds216 to 227/RTCIAT899_ PB01300 to RTCIAT899_ PB01345/nodABCSUIJHPQ1Q2 | NF production | 8.73 to 1.28 | 13.66 to 2.54 |
2 (250 pb) | cds177 to 180/RTCIAT899_ PB01095 to RTCIAT899_ PB01110/nodA2hsnTnodFE | NF production | 10.3 to 10.37 | 9.81 to 11.94 |
3 (180 pb) | cds450 to 449/RTCIAT899_ PB02710 to RTCIAT899_ PB02705/nodM | NF production | 2.43 to 3.18 | 5.85 to 8.49 |
4 (680 pb) | cds95 to 93/RTCIAT899_PB00575 to RTCIAT899_PB00565/y4wEF | Synthesis of IAA | 8.57 to 1.63 | 12.17 to 3.27 |
5 (38 pb) | cds263 to 262/RTCIAT899_PB01550 to RTCIAT899_PB01545/HP | Unknown | 6.75 to 4.26 | 28.65 to 14.37 |
6 (70 pb) | cds61 to 62/RTCIAT899_PB00370 to RTCIAT899_PB00375/HP | Unknown | 1.80 to 2.11 | 2.17 to 1.64 |
7 (191 pb) | cds170/RTCIAT899_PB01055/HP | Unknown | 1.18 | 5.68 |
8 (470 pb) | cds81/RTCIAT899_PB00495/HP | Unknown | −1.05 | 2.58 |
9 (979 pb) | cds173/RTCIAT899_PB01570/nodD2 | Transcriptional regulation | −1.25 | 3.01 |
10 (292 pb) | cds89/RTCIAT899_PB00540/HPa | Unknown | 1.13 | −1.44 |