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Fig. 1 | BMC Genomics

Fig. 1

From: An iNTT system for the large-scale screening of differentially expressed, nuclear-targeted proteins: cold-treatment-induced nucleoproteins in Rye (Secale cereale L.)

Fig. 1

Vectors and screening efficiency of the iNTT system. a Yeast cells transformed with pLexAD. b Yeast cells transformed with pLexAD-NES. c Yeast cells transformed with pLexAD-NLS. d Yeast cells transformed with pLexAD-NES-NLS. e Yeast cells transformed with pLexAD-GmAREB. f Yeast cells transformed with pLexAD-NES-GmAREB. g The expression cassettes used in the NTT system. PADH1: yeast alcohol dehydrogenase 1 (ADH1) gene promoter; LexA: DNA-binding domain of the LexA protein; GAL4AD: transactivation domain of the GAL4 protein; TDAH1: terminator of the ADH1 gene. h The expression cassettes used in the iNTT system. i The expression cassettes used in the iNTT system fused to GmAREB. j The number of yeast clones transformed with the four vectors pLexAD-NLS, pLexAD-NES-NLS, pLexAD-GmAREB, and pLexAD-NES-GmAREB

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BMC Genomics

ISSN: 1471-2164

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