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Fig. 1 | BMC Genomics

Fig. 1

From: Efficient identification of CRISPR/Cas9-induced insertions/deletions by direct germline screening in zebrafish

Fig. 1

CRISPR sgRNA design process. An Ensembl gene model is shown at the top; two exons are expanded in the rest of the diagram. The target sequence is searched for CRISPR sites, these are scored and the best scoring are selected. For screening, PCR primers are designed for making amplicons into sequencing-ready libraries. The package also includes a database schema for storing information on CRISPR designs and screening information

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BMC Genomics

ISSN: 1471-2164

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