Fig. 3

Selected gene expression by Holstein and Angus fibroblasts in response to LPS. Differences in expression of TLR4 (a) IL8 (b) TNF (c) CCL20 (d) and CCL5 (e) between Holstein and Angus fibroblast cultures at hours 0, 2, and 8 post-LPS exposure were determined by RT-qPCR. Values are expressed as delta Ct, or the difference in cycles to threshold (Ct) between the gene of interest and the endogenous gene control beta-actin (ACTB). Fold differences (2^-ΔΔCt) in expression between Holstein and Angus cultures are indicated in parentheses. All values are mean ± SEM. (n = 4/group). *P < 0.05 and **P < 0.01 (t-test)