Table 1 Average response to LPS from combined Holstein and Angus cultures measured by increases in expression of immune-associated genes compared to hour 0 post-LPS

Transcription and activation pathways

Hour 2

Hour 8

BIRC3

Baculoviral IAP repeat-containing protein 3

7.3

4.2

NFKBIA

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha

24.3

9.3

NFKBIZ

Nuclear factor of kappa-B inhibitor zeta

11.7

3.2

NFKB2

Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2

- -

2.8

NFKBID

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, delta

2.3

- -

Cytokines, chemokines, and growth factors

CCL2

Chemokine (C-C motif) ligand 2

14.9

14.6

CCL5

RANTES

49.6

132.8

CCL20

Chemokine (C-C motif) ligand 20

1102.1

3147.3

CXCL2

Chemokine (C-X-C motif) ligand 2

67.9

31.6

CXCL6

Chemokine (C-X-C motif) ligand 6

12.3

45.0

IL1A

Interleukin 1, alpha

9.2

5.7

IL6

Interleukin 6

88.2

124.1

IL8

Interleukin 8

325.7

387.8

SAA3

Serum Amyloid A 3

65.1

1145.6

TNF

Tumor Necrosis Factor, alpha

87.4

- -

Type I IFN-related genes

IRF1

Interferon regulatory factor 1

12.3

4.2

IRF5

Interferon regulatory factor 5

3.0

- -

ISG15

Ubiquitin-like modifier

- -

308.3

MX2

Myxovirus (influenza virus) resistance 2

- -

156.6

OAS1

2'–5'- oligoadenylate synthetase 1

- -

128.7

OAS2

2'–5'- oligoadenylate synthetase 2

- -

65.6