Fig. 6
Ex vivo theLiTE™ assay of Aspergillus nidulans metabolite extracts from ionic liquid supplemented media. Microscope image of Drosophila egg chambers showing presence/absence of the polarity marker protein Par6 when exposed to solvent only solution (0.6 % DMSO, i.e. blank) and the-103, respectively (a); % of egg chambers showing Par6 ring impairment in the presence of 24 μg/mL monodictyphenone, orsellinic acid, the-103 (the blank is also shown) (b) or metabolite extracts collected at incubation day seven in the control medium (ME-c) or either choline (ME-ch) or 1-ethyl-3-methylimidazolium chloride ([C2mim]Cl) (ME-cm) supplemented media, before (grey bars) or after dereplication of its polar fraction (dME) (black bars) (c). The asterisk denotes a significant difference (p-value ≤ 0.05) of each treatment compared to the control