Fig. 10

Functional consequences of gene expression variation between npr-1(ur89) and N2 upon pathogen exposure. a Enrichment of gene ontology (GO) terms. The shown terms were significant with FDR < 0.05 (Additional file 17). b Overview of enrichment of pathogen- and stress-induced gene sets, inferred from EASE analysis on the various clusters of co-regulated genes. The significantly enriched gene sets are indicated on the top and include - from left to right - differentially expressed genes upon exposure to (i) B. thuringiensis Cry5B toxin [89]; (ii)-(iii) the same B-18247 strain used in the current study in C. elegans isolate MY15 or MY18 [90]; (iv)-(v) the same PA14 strain used here [66, 91]; (vi) Oxidative stress response [92]; (vii)-(ix) Osmotic induction [93]; (x)-(xi) Heavy metal Cadmium dysregulated genes [89, 94]; (xii) Pesticide influence [58]. c Overview of enriched gene sets for selected immunity pathways and general categories, including (i)-(ii) the p38 MAPK pathway (pmk-1 and sek-1 targets; [91]); (iii)-(v) insulin signalling (daf-2 targets; [95–97]); (vi) npr-1 targets [18]; (vii) Glycoproteins; (viii)-(x) Cytochrome P450 [98–100]; (xi) Protein kinase [98]; (xii) Lipid metabolism [98]; (xiii) Cell division [98]. d Enrichment of Ebox and GATA motifs and their transcriptional targets. The enriched gene sets were inferred with EASE and are indicated at the top, including differentially expressed genes in mutants of (i)-(iii) E-box transcriptions factors [101] or (iv)-(v) GATA transcription factors ELT-2 and ELT-3 [102, 103]. Enriched transcription factor binding motifs were inferred with AMD and are shown on the right (Additional file 17). In all panels, the clusters are given on the very left and are identical to those in Fig. 9. Red color indicates an enrichment for up-regulated genes per gene set, blue that for down-regulated genes per gene set. Color intensity corresponds to the significance level, inferred by EASE analysis (see scale at the right side)