Fig. 3

miR-9 sequence heterogeneity. a Significant sequence variation was detected among mature miRNA reads. The figure shows a compilation of miR-9 variants; the extent of variation at any particular nucleotide is indicated by the size of the font by WebLogo [74]. b (Below) The mature 5p miRNA sequence is shown in green, and the 3p miRNA sequence is shown in red; the adjacent genomic sequence is shown in black. Single nucleotide substitutions are highlighted in gray. The number of unique reads that are aligned to the putative precursor is listed on the right side of the figure. c A graphic illustration of the hairpin-loop precursor is shown on the right of the figure. The dominant cleavage sites are indicated using black arrows. Large arrows show the most abundant isomer cleavage sites, whereas small arrows indicate less abundant isomiR cleavage sites. The most abundant mature miRNAs (5′p and 3′p) are shown by the regions shown in green and red