Fig. 6

Involvement of V-H⁺-ATPase during de-etiolation of tomato seedlings. a Analysis by qPCR of V- ATPase subunit c2 (B-D5) during de-etiolation. The data represent the average fold change of 3 independent biological replicates ± SEM. Normalization was done using the pp2ase gene as housekeeping gene. Fold change was calculated compared to the value obtained for the dark control sample. The non-parametric Mann-Whitney U test (Statistica 12) was used to determine the significance of the results. b Analysis by qPCR of VHA-A1 subunit during de-etiolation. The data represent the average fold change of 3 independent biological replicates ± SEM. Normalization was done using the pp2ase gene as housekeeping gene. Fold change was calculated compared to the value obtained for the dark control sample. The non-parametric Mann-Whitney U test (Statistica 12) was used to determine the significance of the results. c Effect of bafilomycin A1 on hypocotyl growth of tomato seedlings grown in BL . Germinated seeds were grown either in darkness or under constant BL on Murashige and Skoog medium containing varying concentrations of BafA1. After 5 days of growth, the length of hypocotyl was measured with a ruler to the nearest millimeter. The data are presented as boxes and whiskers. The whiskers represent the range of the data; the white dot within the box indicates the median value, while the boxes’ lower and upper boundaries indicate the first and third quartiles, respectively. An average of 45 plantlets coming from independent replicates was measured. The non-parametric Kruskal-Wallis Anova with multiple comparison of mean rank was used for statistical significance of the data (Software: Statistica 12); a: statistically different from the control condition with p-value ≤ 0.01