Table 1 Primers used in quantitative real-time PCR
Identification | Description of the gene | Primers | Primer efficiency |
|---|---|---|---|
A-E4 | Mitogen-activated protein kinase | F: 5′- GAAGATGAGAAACCACAAGCG | 90 % |
R: 5′- CATTCTGAGGAACTTGGAGAGG | |||
C-G1 | Importin subunit alpha1a | F: 5′- GAACTCATTTTGTGCCCCATC | 92 % |
R: 5′- GCTGAGGGATTGGAAAAGATTG | |||
E188 | Intracellular Ras-group-related LRR protein 9 | F: 5′- GAGAGGCAGGATTGGAGATTG | 94 % |
R: 5′- TCCGCATCCTTCAACATCTTC | |||
E-E3 | Polyadenylate-binding protein RBP47 | F: 5′- TCCTAATGAGCCTAACAAACCTG | 92 % |
R: 5′- TCCGTCTTATTGCCTTCCAC | |||
VHA-A1 | V-ATPase catalytic subunit A1 | F: 5’- CGAGAAGGAAAGCGAGTATGG | 107 % |
R: 5’- TCATTCACCATCAGACCAGC | |||
B-D5 | Vacuolar H + -ATPase V0 sector | F: 5′- GCAGTCATTATCAGTACCGGG | 89 % |
R: 5′-TCTAACACCAGCATCACCAAC | |||
B-E2 | Pectin acetylesterase | F: 5’-CACACCCACAAAGAGAAACAG | 103 % |
R: 5’-TTCCAAGAATGCCCCTTCAG | |||
12. | XTH | F: 5’-AGAGGTGGGCTTGAGAAAAC | 93 % |
R: 5’-GAACCCAACGAAGTCTCCTATAC | |||
B-D9 | 26S proteasome | F: 5’-TCTTGTCCTCTTTCTGTTCCTTATC | 95 % |
R: 5’-AATCCTTGCCTCACTTCCAG | |||
C-A3 | Histone H2B | F: 5′- TTGGTAACAGCCTTAGTTCCTC | 89 % |
R: 5′- AAAGCCTACCATCACTTCTCG | |||
PP2ACS | PROTEIN PHOSPHATASE 2A catalytic subunit | F: 5’- CGATGTGTGATCTCCTATGGTC | 98 % |
R: 5’- AAGCTGATGGGCTCTAGAAATC |