Fig. 3

Representation of DEG between pairwise sample comparisons of the four populations in chronological order. The following comparisons were performed: 2C/LZ; LZ/PS; PS/RS (|FC| ≥ 2; Kal’s test p ≤ 0.01). a. Heat map of expression levels and hierarchical clustering for the global gene expression in the four samples. All genes detected as differential in at least one sample were included. Z-score values are coded on the green-to-red scale (high expression: red; low expression: green). b. Venn diagram of up-regulated and down-regulated genes. Separate and overlapping expression between samples is shown. c. Temporal expression profiles of DEG, ordered based on the p-value significance of the number of assigned vs expected genes. Only the 10 most significant profiles are shown. The p-value (bottom of each panel) and number of genes (below) for each profile are shown