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Fig. 6 | BMC Genomics

Fig. 6

From: Transcriptome analysis of highly purified mouse spermatogenic cell populations: gene expression signatures switch from meiotic-to postmeiotic-related processes at pachytene stage

Fig. 6

Dynamic expression patterns of 13 selected genes representative of the different expression profiles. The analyses were carried out by RNAseq (CLC bio and edgeR) and qPCR. a. Expression profiles obtained by RNAseq analysis (CLC bio: orange; edgeR: blue) and qPCR analysis (yellow). Col1a1: collagen, type I, alpha 1; Sycp3: synaptonemal complex protein 3; Dazl: deleted in azoospermia-like; Tex15: testis expressed 15; Top2a: DNA topoisomerase II, alpha isozyme; Dnahc8: dynein, axonemal, heavy chain 8; Tcte3: T-complex-associated-testis-expressed 3; Atp8b3: ATPase, aminophospholipid transporter, class I, type 8B, member 3; Clgn; calmegin; Spa17: sperm autoantigenic protein 17; Ldhc: lactate dehydrogenase c; Tnp1: transition protein 1; Prm1: protamine 1. b. Correlation between the expression levels in RNAseq analysis (RPKM values of CLC bio analysis) and those obtained by qPCR analysis for the 13 selected genes in the four testicular cell populations

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