Fig. 3

Relationship between prediction and measurement for bloodstream and procyclic forms. For each unique gene considered, the amount of mRNA that was measured was divided by the predicted amount, and the log₂ of the values for both stages plotted. BS - bloodstream form, model BS-D; PC - procyclic form, model PC-A. A value of 0 indicates that the prediction was perfect; this could however also be true if transcription was faster, and processing slower, than the values that were set in the model. Measures above 0 indicate either that a processing half-time is shorter than 1.7 min, or that the steady-state transcription rate is greater than the value that was used for the model. values below 0 indicate that the splicing or polyadenylation half-time is longer than 1.7 min. Spots above the line “PC = 2xBS” are mRNAs for which the measured/prediction results was at least 2-fold higher in procyclic than in bloodstream forms; those below the “BS = 2xPC” line have better processing in bloodstream forms than in procyclics