Fig. 3

Colony PCR results indicating that group II intron-based vectors successfully targeted the Serratia sp. LCN16 oxyR and kat genes. a Introns were inserted in the position 614|615 of oxyR gene, oxyR::int(614); and in the position 808|809 of katA gene, katA::int(808). For both genes, the arrows indicate the position of forward and reverse primers designed to infer intron insertion. b L1 and L4 indicate 100-bp and 1-kb molecular markers, respectively. L2 and L5 correspond to the oxyR (846 bp) and katA (292 bp) fragments. L3 and L6 correspond to mobile group II intron integrated in oxyR (about 3Kb) and katA (about 2-3Kb), respectively