Fig. 3

Mpdz expression and MUPP1 levels in mouse spinal cord tissue. In these experiments the effects of both genotype and morphine treatment were evaluated. Panel (a) provides the results of qPCR experiments in which the index C57BL/6 and low-adapting 129S1/SvIm strains were used. Baseline Mpdz levels were lower in the 129S1/SvIm strain, but the levels of Mpdz mRNA were unchanged by morphine treatment in both of the strains tested. In panel (b) the results of Western analysis are provided demonstrating that 129S1/SvIm mice have lower spinal levels of Mpdz/MUPP1 protein. For each trait the mean value is displayed +/− S.E.M., n = 6 mice per group. *p < 0.05, ***p < 0.001