Fig. 5

Normalized K cluster analysis of all genes compared to connectivity genes only shows improved resolution of expression differences. a Analysis of all genes (n = 31,860), K cluster groups shown as heat maps, in which gene expression is averaged across development, and deviations both from the developmental average, and hypoxia versus normoxia, are shown. Values in parentheses indicate log2 range fold change of genes in the cluster. b Examples of differential assignment of genes to different clusters (heat-maps of connectivity genes is from Fig. 4a). Green lines show different groups of genes assigned to different clusters in the two analyses for cluster 13. Red lines show differential assignment for cluster 5. c Table representation of differences in gene assignment to K clusters, comparing all genes (columns) to connectivity genes only (rows)