Fig. 1

Experimental design of microRNA analysis of lizard tail regeneration. a: Image of a green anole lizard with a fully regenerated tail (arrow at break point). b: A 25 dpa regenerating tail was divided into three equally sized segments, with the distal regenerating tip and proximal regenerating base collected for microRNA sequencing (sequenced each for the regenerating tail tip and base, n = 3 per pool). For qRT-PCR analysis, five equally sized segments were collected (n = 4). c: Venn diagram showing the distribution of microRNAs expressed in the brain, skeletal muscle, and 25 dpa regenerating tail tip and base (minimum count of 1)