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Fig. 4 | BMC Genomics

Fig. 4

From: A robust (re-)annotation approach to generate unbiased mapping references for RNA-seq-based analyses of differential expression across closely related species

Fig. 4

qPCR results. Boxplot of normalized Ct values (reference gene: actin 79B) For each studied gene (one colour) boxplot is showed for Ct values in D. melanogaster OreR (“D. mel”) and D. mauritiana TAM16 (“D. mau”). (Significance calculated by t-test (for genes with homogeneous distribution of variances) or t-Welch-test (for genes with not homogeneous distribution of variances); *p < 0.05, **p < 0.005; ***p < 0.0005)

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