The Tetrahymena metallothionein gene family: twenty-one new cDNAs, molecular characterization, phylogenetic study and comparative analysis of the gene expression under different abiotic stressors

Table 2 Distribution of Cys clusters among Tetrahymena MTs

CdMT	CCC	CXCC	CXCXC	XCCX	CXC	XXCXX	Total Cys	% Cys^(a)	Length
TpigMT-1	4	2	0	6	2	0	34	28.81	118
TpyrMT-1	4	2	0	5	1	1	31	28.97	107
TpyrMT-2	6	5	0	6	4	1	54	29.83	181
TtheMTT1	6	3	0	8	2	1	48	29.63	162
TtheMTT3	2	2	1	9	3	3	42	25.93	162
TtheMTT5	1	1	0	5	1	6	24	24.24	99
TrosMTT1	4	2	0	6	2	0	34	30.09	113
TtroMTT1	6	3	0	8	2	0	47	30.13	156
TmobMT1	6	3	0	9	3	0	51	29.65	172
TvorMT1	6	3	0	8	2	1	48	28.40	169
ThegMT1	4	2	0	6	2	0	34	27.87	122
ThegMT2	4	1	0	8	7	4	49	25.65	191
ThegMT3	6	3	0	9	3	0	51	28.65	178
ThegMT4	5	1	0	8	6	4	50	25.91	193
TborMTT1	4	2	0	6	2	0	34	30.09	113
TborMTT2	5	2	2	9	1	2	49	30.06	163
TelliMTT1	6	3	0	7	1	2	45	29.22	154
TelliMTT2	6	3	0	6	0	3	42	28.97	145
TmalaMTT1	6	3	0	8	2	1	48	29.63	162
TmalaMTT2	5	3	0	8	1	2	44	27.16	162
TmalaMTT3	2	2	0	12	2	6	46	25.70	179
TmalaMTT4	1	1	0	7	1	3	25	25.25	99
TpatMTT1	4	2	0	6	2	0	34	29.06	117
TpatMTT2	4	2	0	6	3	3	39	26.35	148
TamerMTT1	4	2	0	5	1	1	31	29.25	106
TamerMTT2	6	3	0	8	2	1	48	30.57	157
Total Cys^(b)	351	183	9	378	116	45	1082	28.27	147
% Cys ^(c)	32.4	16.9	0.8	34.9	10.7	4.1	100
CuMT
TpigMT-2	0	0	0	1	12	2	28	29.17
TtheMTT2	0	0	0	0	15	2	32	29.63	96
TtheMTT4	0	0	0	0	15	2	32	29.63	108
TrosMTT2	0	0	0	1	9	2	22	28.21	108
TtroMT1	0	0	0	1	12	2	28	28.00	78
TtroMT2	0	0	0	0	15	2	32	26.63	100
sp1.7-MT1	0	1	0	0	14	1	32	26.63	108
TelliMTT6	0	0	0	0	21	2	44	29.73	108
TelliMTT8	0	0	0	0	12	2	26	28.89	148
TmalaMTT5	0	0	0	0	15	2	32	29.63	90
TborMTT3	0	0	0	1	21	0	44	27.85	108
TborMTT4	0	0	0	1	6	2	16	26.67	158
TborMTT6	0	0	0	1	21	0	44	27.85	60
TborMTT7	0	0	0	0	21	0	42	28.19	158
TborMTT8	0	0	0	1	9	2	22	28.21	149
TamerMTT3	0	0	0	0	14	3	31	28.97	78
ImMTT2	0	0	0	0	27	0	54	32.34	107
Total Cys^(b)	0	3	0	14	518	26	561	28.60	167
% Cys^(c)	0	0.5	0	2.5	92.3	4.6	100		113

New MTs reported in this study are in bold text. A cluster is defined as any group of contiguous residues in which any two Cys residues are separated from one another by, at most, any other amino acid (X). ^(a) Percentage of Cys residues in the complete MT. ^(b) Total Cys residues/cluster type. ^(c) Percentage of Cys residues/cluster type

ISSN: 1471-2164