Fig. 6

Evolution during development, fold change values between small and large berries, and selected cis-regulatory element profile of cell wall transcripts differentially expressed at 47, 74, 103, and 121 DAA. The heat map represents the evolution (a and c), based on the mean log2 (FPKM + 1) of small and large berries, and log2 fold (small/large) changes (b and d) of cell wall degradation (a and b) and modification (c and d) genes. The relative log2 (FPKM + 1) values registered in small and large berries on average during berry development in a and c are depicted by green (high expression) and blue (low expression). Grey color indicates the absence (or low levels) of detectable transcripts at the corresponding stage. Blue and red boxes in b and d indicate downregulated and upregulated transcripts, respectively, in small berries in relation to large berries. Boxes with bold margins indicate significant differences (adjusted P-value <0.05) between small and large berries at a given developmental stage. The cluster column in b and d indicates the cluster number the associated transcript belongs to. EM, 1,4-beta-mannan endohydrolase; PL pectate lyase; PG Polygalacturonase; XET/XTH Xyloglucan endotransglucosylase/hydrolase. (e and f) The heat map illustrates the presence of AP2/ERF and bHLH/NAC cis regulatory elements in cell wall genes. Purple and white colors depict the presence and absence of the respective CRE in the promoter regions of the gene