Fig. 3

Comparisons of gene expression from microarray and qRT-PCR analysis. Empty bars represent the Log2 ratio obtained from microarray analysis and filled bars represent this value obtained by qRT-PCR. †: P < 0.10; *: P < 0.05; **P < 0.01; ***P < 0.001. a Log2 ratios obtained by comparing chickens thermally manipulated during embryogenesis and reared in standard conditions (TM) to control chickens incubated and reared in standard conditions (C). b Log2 ratios obtained by comparing control chickens submitted to a heat challenge at day 34 (CHC) to C chickens. c Log2 ratios obtained by comparing TM chickens submitted to a heat challenge at day 34 (TMHC) to TM chickens. EME2, essential meiotic endonuclease 1 homolog 2; COQ6: coenzyme Q6 homolog, monooxygenase; MCAT: malonyl CoA:ACP acyltransferase; MRPL28: mitochondrial ribosomal protein L28; ADHFE1: alcohol dehydrogenase, iron containing, 1; TMEM111: transmembrane protein 111; TBCE: tubulin folding cofactor E; B-G: V-region-like B-G antigen-like; SCN5A: sodium channel, voltage-gated, type V, alpha subunit, transcript variant 4; CIB2: calcium and integrin binding family member 2; PTEN: phosphatase and tensin homolog; TTN: titin; OBSCN: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF; HSP90AA1: heat shock protein 90 kDa alpha (cytosolic), class A member 1; PDK4: pyruvate dehydrogenase kinase, isozyme 4; SERPINH1: serpin peptidase inhibitor, clade H (heat shock protein 47), member 1; UBB: ubiquitin B; COL12A1: collagen, type XII, alpha 1; DUSP8: Dual specificity phosphatase 8; ARRDC4: arrestin domain containing 4; ENC1: ectodermal-neural cortex 1; HDAC1: histone deacetylase 1; BIRC5: baculoviral IAP repeat-containing 5 (survivin); HSP70A2: Heat shock protein 70 A2; SIRT1: sirtuin 1; POLR2D: polymerase (RNA) II (DNA directed) polypeptide D; SPP1: secreted phosphoprotein 1; TXNRD2: thioredoxin reductase 2