Fig. 3

ATF3 globally binds genomic sites enriched with p300 and H3K27ac. a Genome browser views showing co-localization of ATF3 with p300 and H3K27ac in several representative genomic sites. b Venn diagram showing overlaps between ATF3 peaks and p300 peaks. c Intensity plot showing co-localization of ATF3 with p300 and H3K27ac. d Venn diagram showing overlaps between p300 distal peaks (active enhancers) and ATF3 peaks. e ChIP-qPCR validation of p300 binding to the ATF3 binding sites. HCT116 cells were subjected to ChIP using a p300 antibody. Precipitated DNA was quantitated using qPCR. f H3K27ac was enriched in the ATF3-binding sites. ChIP-qPCR was carried out to determine H3K27ac levels in the ATF3-binding sites