Fig. 4

Binding of ATF3 to active enhancers correlates with ATF3-regulated gene expression. a Heatmaps showing ATF3-regulated genes, and their binding by ATF3 and p300. b qRT-PCR validation of genes differentially expressed between ATF3-wildtype and knockout cells identified by microarray. c, d Validation of ATF3 binding to differentially-expressed genes by ChIP-qPCR. e ATF3 was localized in regions distal to TSS (>2 kb) of differentially-expressed genes. f Representative genome browser views of co-localization of ATF3 and p300 in ATF3-regulatd genes. g ATF3 was localized in active enhancers of ATF3-regulated genes. Error bars represents SD