Perturbation of the two-component signal transduction system, BprRS, results in attenuated virulence and motility defects in Burkholderia pseudomallei

Table 1 Genes with differential expression in the bprRS double mutant compared to expression in the wild-type parent strain

Locus tag	Gene product/description	bprRS mutant Expression (log₂)	FDR
Increased expression in bprRS double mutant relative to wild-type expression
BPSS0686	putative 5, 10-methylenetetrahydro-methanopterin reductase	3.36	3.21E-07
BPSS0686a	protein of unknown function	5.63	7.35E-05
BPSS0687	BprS-sensor kinase protein	4.34	1.67E-07
BPSS0688	BprR-response regulator protein	2.07	1.31E-05
BPSS1087	phage membrane protein	1.13	3.41E-03
Decreased expression in bprRS double mutant relative to wild-type expression
BPSL0145	protein of unknown function with ATPase domain, putative phage-encoded	−5.17	1.17E-04
BPSL0146	membrane protein	−3.48	9.28E-06
BPSS1066	phage terminase, endonuclease subunit	−3.62	2.86E-03
BPSS1072	phage-acquired protein	−4.89	9.28E-06

Genes were identified as differentially expressed if they showed >2-fold expression change (log₂ > 1.0 or log₂ < −1.0) compared to the wild-type strain with a False Discovery Rate (FDR) of <0.01

ISSN: 1471-2164