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Table 4 Putative de novo SNPs present in the lab-adapted C. remanei population above 6 %

From: High-specificity detection of rare alleles with Paired-End Low Error Sequencing (PELE-Seq)

Position Ref Alt Wild allele frequency Lab allele frequency Wild depth Lab depth
8678151 T C 0 0.07 998 993
22410779 C T 0 0.16 992 997
23864162 T A 0 0.06 991 895
27788600 A G 0 0.069 995 956
67266085 C T 0 0.066 998 846
67492174 A G 0 0.07 940 961
96566683 T C 0 0.071 988 971
127028996 C T 0 0.065 982 965
143968069 T G 0 0.121 996 988
  1. Many putative de novo SNPs were present at significant frequencies in the lab-adapted population, despite being absent in the wild population. Using a minimum read depth of 900× overlapping paired-end (OPE) reads, PELE-Seq detected 9 putative de novo SNPs found above 6 % frequency in the lab-adapted population