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Fig. 2 | BMC Genomics

Fig. 2

From: Next-generation sequencing-based detection of germline L1-mediated transductions

Fig. 2

Computational analysis of the chr7:6620368-6620628 insertion into the chr10:54643580-54643593 region in the chimpanzee sample PR01171. a Depiction of the chr10:54643580-54643593 region using the Integrative Genomics Viewer (IGV) [57] before read realignment (upper panel). After realignment using BLAT many initially single-anchored reads were placed correctly, facilitating the ascertainment of this L1-mediated transduction clustering to a region on the source chromosome 7 with an average uniqueness of 1 (reads mapping exactly once to the reference genome). b A detailed view of L1-mediated 3′ transduction read placements: one read is shown to map to the target locus on chromosome 10 and the other read (mate of the pair) maps either to a non-reference L1 element (displayed on the top panel) or forms a cluster of reads uniquely mapping to the source on chromosome 7 (displayed on the lower panel). Out of 29 reads, 7 were carrying parts of a non-reference polyA tail (only subset of reads shown)

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