Fig. 3

Significant pathways altered by in-ovo thermal modifications; in (a) embryonic stage and (b) D35. DEGs associated with each comparison (treatment vs control) are separated into up-regulation (U) or down-regulation (D). Thermal modification treatments: increase (H) or decrease (L) incubation temperature during E7-10 (H10 and L10) or E10-13 (H13 and L13). Significant pathways (IPA defined) are grouped into eight major categories of interest; group (gr.)1 cell maintenance proliferation differentiation and replacement, gr.2 organismal organ and tissue development, gr.3 nutrient metabolism, gr.4 genetic information and nucleic acid processing, gr.5 molecular transport, gr.6 cell signaling and interaction, gr.7 small molecule biochemistry, and gr.8 response to stimuli and associated. The –log (BH p-value) associated with significant pathways are plotted in green (small) to red (large)