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Fig. 1 | BMC Genomics

Fig. 1

From: In vitro infectivity and differential gene expression of Leishmania infantum metacyclic promastigotes: negative selection with peanut agglutinin in culture versus isolation from the stomodeal valve of Phlebotomus perniciosus

Fig. 1

Isolation of Pro-Pper. Promastigotes within the stomodeal valve of P. perniciosus. Sand flies were dissected and the guts separated. The abdominal gut and the posterior part of the thoracic midgut were discarded and Pro-Pper were recovered from the anterior part of the thoracic midgut (discontinuous line). The Pro-Pper population is enriched in metacyclic promastigotes as they are near the stomodeal valve (SV). Carryover of leptomonads was minimized by recovering just promastigotes in suspension and avoiding gut tissue as much as possible. However, it is assumed that this population is heterogeneous as expected in any biological experiment, as well as the Pro-PNA− population

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BMC Genomics

ISSN: 1471-2164

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