Fig. 5

Confirmation of thymic DETs by qPCR. The qPCR analysis was conducted on ten genes (TLR4, TLR15, AVD, IL8L2, BPI, SOCS3, IL6ST, IL1R2, HSPB1 and NOV) at 8 time points including 0, 2, 6, 12, 24, 36, 72 and 120 h after LPS challenge. Statistically significant differences for multiple comparisons (12 hpt vs. 0 hpt, 36 hpt vs. 0 hpt, 72 hpt vs. 0 hpt) were performed with Bonferroni’s multiple comparisons test after one-way ANOVA test. *P < 0.05, **P < 0.01, ***P < 0.001. RNA-seq was performed for the thymuses collected at 4 time points including 0, 12, 36, and 72 h after LPS challenge. The P values for transcript expression differences between different time points from RNA-seq were determined using MARS method. ^# P < 0.001. Results are presented as means ± SD (n = 3)