TY - JOUR AU - Gillen, Austin E. AU - Yamamoto, Tomomi M. AU - Kline, Enos AU - Hesselberth, Jay R. AU - Kabos, Peter PY - 2016 DA - 2016/05/05 TI - Improvements to the HITS-CLIP protocol eliminate widespread mispriming artifacts JO - BMC Genomics SP - 338 VL - 17 IS - 1 AB -  High-throughput sequencing of RNA isolated by crosslinking immunoprecipitation (HITS-CLIP) allows for high resolution, genome-wide mapping of RNA-binding proteins. This methodology is frequently used to validate predicted targets of microRNA binding, as well as direct targets of other RNA-binding proteins. Hence, the accuracy and sensitivity of binding site identification is critical.  SN - 1471-2164 UR - https://doi.org/10.1186/s12864-016-2675-5 DO - 10.1186/s12864-016-2675-5 ID - Gillen2016 ER -