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Fig. 5 | BMC Genomics

Fig. 5

From: Improvements to the HITS-CLIP protocol eliminate widespread mispriming artifacts

Fig. 5

miR-888 overexpression represses Progesterone Receptor expression. a. Luciferase reporter assays confirm functional binding of overexpressed miR-888-3p to PGR-MRE and disruption of this interaction upon mutation the seed-binding region. b. Western blot analysis of BT474 cells transfected with miR-888 or control (5 nM each). MiR-888-3p overexpression represses PGR expression as compared to cells transfected with control mimic. c Progesterone treatment increases the number of CK5 expressing cells in ER+ breast cancer cells (T47D are shown). MiR-888-3p overexpression effectively blocks the progesterone induced increase of CK5

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