Fig. 5

Evidence of a multi-operon organization within PUL 43. a Read coverage (in grey) within PUL 43 region and read information for that region, colored by strand, are given for the three RNA samples obtained with OSX condition. The total coverage of the genome in that region is extremely high, about 1000-4000× and was not scalable. Hence, the displayed reads were down-sampled in the window to ~50× depth. The black bars just below the grey coverage histogram for each sample indicate regions where down-sampling was performed. b Gene organization of PUL 43 with the position number of the neighboring gene regions used as target for the RT-PCR experiments. The intergenic distance between two genes is given in the table. c Operons predicted from genomic data using the FGENESB tool (see Methods). d Operons predicted from RNA-seq data using RockHopper (see Methods). e Operons experimentally validated by RT-PCR, by amplifying the intergenic regions between two consecutive genes. f The detection of RT-PCR fragments is shown on the agarose gel and the expected sizes of each product are given in the table