Table 3 Primers of qRT-PCR assay used for eight RNA-seq libraries in this study
From: Transcriptome analysis revealed the drought-responsive genes in Tibetan hulless barley
Gene_id | Amplicon size (bp)a | Forward primer (5′- > 3′) | Reverse primer (5′- > 3′) |
|---|---|---|---|
HVU014442.1 | 142 | ATTTCTTCGACTGGGGCCTG | ATGACCTTGCCGTCGATCTC |
HVU014441.1 | 190 | CAGGAGCCTGAGTAGATGCG | AATGAGAGGCCGACCACAAG |
HVU029679.1 | 194 | AAGAAACTGATCCGAGGCGG | ATTCGTCCGGCCCGTATTTT |
HVU021835.1 | 180 | GCGGCTATATCCCACCTTCC | AAGAGCTGAGGTGAAGCGAC |
HVU015180.1 | 208 | TGGAGAAACGGATCGAAGCC | TGCAGCCCAGCTAGAAAAGG |
HVU021802.1 | 206 | CCTTCACCTCCAGGAACGTC | CACTCCGATTCCACTTGGCT |
HVU025938.1 | 180 | TGTCACCGCTGAACCAATCA | TTCACTGGGATTCACGGACG |
HVU026759.1 | 86 | GAAGTCGCGCGAATCTGTTC | GAGCAGAGCAGCCAGATCAA |
HVU015112.1 | 215 | AAGATCCCAACAAGGCGAGG | CCTGGCTTGCTCCTCTTTGA |
HVU001215.1 | 229 | AAGCATGCCGTCTTACACCA | GGCCATCGGAGAGTGCATTA |
HVU012352.1 | 163 | TGGCGTGTCAGCTGAGATTT | GGCCGTGAAGGACCAAAAAC |
HVU033128.1 | 214 | CCATCAGACTGTGGCGTCTT | GAACCCTCTGCGCATAGACA |
HVU023469.1 | 244 | AGCACTACTACGGCACCAAC | CTGAAACCGGCGTACTCCTT |
HVU007307.1 | 216 | AGATGGGGTGCGAACTTGAG | CAGGGACAGGACCATCCAAC |
HVU011039.1 | 115 | GGTGATCATGCGTGTGTTCG | CTTCTTGGCCGAGTCCTTGT |
HVU033209.1 | 234 | AGCCCAAACCTACCAAGCTC | ATCATAGCGCAGGAGCCATC |
HVU000918.1 | 87 | GTAGCTATCCACGGTCACGG | TGGAACTGTAAGCGTCCACC |
HVU007765.1 | 208 | CGCATGCTGATGGAAAAGGG | ATTGCACCGCACTCAACAAC |
HVU006724.1 | 135 | TTTGTGTGGGGAGGTCGATG | CATGCACTCTTCGGTGACCT |
HVU016310.1 | 245 | TGAGGATGAAGCGAGTGCAG | TGGGGACTAGCACGCAAAAT |