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Table 4 Validation of PR gene induction by qRT-PCR

From: Theobroma cacao L. pathogenesis-related gene tandem array members show diverse expression dynamics in response to pathogen colonization

   P. palmivora treatment C. theobromicola treatment
  Gene ID Microarray fold induction qRT-PCR fold induction Microarray fold induction qRT-PCR fold induction
PR-1 s Tc01_g003940 N.S. Transcript not detected N.S. Transcript not detected
Tc01_g034430 N.S. N.S. N.S. N.S.
Tc02_g002380 N.S. N.S. N.S. N.S.
Tc02_g002390 N.S. N.S. N.S. N.S.
Tc02_g002400 N.S. N.S. N.S. 8.3 (p = .001)
Tc02_g002410 125.4 763 (p < .001) 91.3 55.7 (p < .001)
Tc02_g002420 N.S. N.S. N.S. N.S.
Tc02_g002430 N.S. N.S. N.S. N.S.
Tc02_g010380 N.S. N.S. N.S. N.S.
Tc05_g005530 N.S. N.S. N.S. N.S.
Tc09_g000720 N.S. N.S. N.S. N.S.
Tc09_g016580 N.S. N.S. N.S. N.S.
Tc09_g016590 N.S. N.S. N.S. N.S.
Tc10_g000980 N.S. Transcript not detected N.S. Transcript not detected
PR-3 s Tc01_g000770 33.6 70.2 (p < .001) 18.8 13.8 (p = .01)
Tc02_g003890 27.1 Transcript not detected 22.31 Transcript not detected
Tc04_g018100 22.5 5086.0 (p < .001) 8.3 36.7 (p = .019)
Tc04_g018110 29.2 763.2 (p < .000) 11.5 13.7 (p = .041)
Tc04_g018160 63.6 158.4 (p = .003) 73 65.6 (p = .001)
Tc06_g000490 N.S. 3.4 (p = .016) N.S N.S.
PR-4 s Tc00_g012980 N.S. N.S. N.S. N.S.
Tc05_g027210 24.9 1027.7 (p < .001) 14.9 22.7 (p = .01)
Tc05_g027220 11.1 258.9 (p = .001) 6.7 N.S.
Tc05_g027230 N.S. 164.1 (p = .011) N.S. N.S.
Tc05_g027250 N.S. N.S. N.S. N.S.
Tc05_g027320 53.4 29.3 (p = .009) 17.8 8.9 (p = .001)
Tc10_g011130 N.S. 61.5 (p < .001) N.S. N.S.
PR-10s Tc01_g031100 39.7 28.0 (p = .019) 57.2 32.3 (p = .002)
Tc04_g028780 25.5 32.9 (p = .027) 25.5 41.6 (p = .004)
Tc04_g028860 6.02 24.3 (p = .038) 53.4 96.8 (p = .001)
  1. Genes shown as induced by microarray had BH p-values < 0.05. N.S. indicates p-values for fold change were > 0.05. Inductions detected by qRT-PCR were calculated using REST software [96] and represent the average of five pathogen-treated samples compared to five water-treated samples relative to TcTub1 (Tc06_g000360). Transcripts were considered undetected if the average Ct value across all treatments was greater than 35