Table 3 Primers used for qRT-PCR validation
Unigenes | KEEG Annotation | Gene | Forward 5'–3' | Reverse 5'–3' |
|---|---|---|---|---|
CL12551Contig1 | Apoptosis | Serine/threonine-protein kinase | TATTCCCTTTTCCGATCTTCGCTAT | CAAAGATGAGTTGCTTTTCGTGTTG |
CL23964Contig1 | Calcium signaling pathway | calcineurin B-like protein | AAATTTGCATATCTGCCTGTGTCAA | ATGTAAAACATCCAAAACCCCAGTC |
CL32645Contig1 | Flavonoid biosynthesis | ANR | AGTATGGCTTTTGTGCTTTGATTGA | TAGAGGTGTCAAGGTTTCAGTTTCA |
CL28991Contig1 | Flavonoid biosynthesis | CYP75A(F3'5'H) | TTGTAAGTATGGGAGTTTGGGTAGG | CGACACATCATCAAGCGTAATAGAG |
CL26013Contig1 | Flavonoid biosynthesis | LDOX | AGTTGGCTAATAATGCTAGTGGTCA | TTTTCTAGTCGGCCTTCTTCTAGTC |
CL20835Contig1 | negative regulation of programmed cell death | Probable WRKY 40 | ATTGTACTTGTCGCAAATGTCTGTT | CAATGTTAACCCACTTCCACTACAC |
CL1Contig2502 | Plant-pathogen interaction | caffeoyl-CoA O-methyltransferase | TCACAAGAACCAGACACAAACATTC | TTAATGGGCACAAGGGTTATGTTTC |
CL41260Contig1 | Plant-pathogen interaction | CPK | GATTCCTAAACATTTCCAAGCCACA | CTTTTAATGGTTGGACGGTGAGAAA |
CL25650Contig1 | Plant-pathogen interaction | PR1 | ACCGTTATTTGTACACTGAACCCTA | TATGCTAATTCGAGGATGGGTGATT |
CL21816Contig1 | Plant-pathogen interaction | RPS2 | CCAATGGGACCTCTGAACTATTTTG | TGCTTAAATTGCCTTTCAACGAAGA |
CL2609Contig1 | Plant-pathogen interaction | CALM | TGAAATCTATCATTCCATCCCCGTT | GAAGGGAGCTTTTGATGTGTTTGAT |
CL1Contig5289 | ubiquitin mediated proteolysis | Skp1 | TGGGTTCCAATGGTATTGTTTGAAG | CGAGTGGATTATCGTTCAGTTGATG |
CL1Contig1054 | ubiquitin mediated proteolysis | Cullin-1 | ATATGTGTGGTAACCCTATTGGTCC | AATTCCCCGTAAACCAGTTCATAGA |
CL25362Contig1 | Response to auxin | Protein TIFY 9 | ATTGACGATTTTCTACAACGGAACC | TCTCCAGAAGTTGATGTTGATCTGT |
CL31783Contig1 | Response to salicylic acid | 12-oxophytodienoate reductase 11 | TCATAACAAGGGTGGGATCTTCTTT | CACTGTATCTTTCATGAACTGGTCG |
CL25983Contig1 | - | Ribonuclease, T2 family | CTGCGATAGCCGCAACTCTT | GGAAGTAGCTGTGCTGGTCAA |
GE651107 | Reference gene | GAPDH | GGAAGCATCATGAACTCAAAGTGAA | ATCCTTCTCATTGACACCCATAACA |