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Fig. 4 | BMC Genomics

Fig. 4

From: Identification and functional characterization of the sulfate transporter gene GmSULTR1;2b in soybean

Fig. 4

Functional expression of GmSULTR1;2b in yeast. (a) YNB solid culture medium containing 0.1 mM homocysteine. (b) YNB solid culture medium containing 0.1 mM sodium sulfate. (c) The position of each yeast strain on the plates. (d) Growth curves generated from a 24 h culture grown in YNB liquid culture medium containing 0.1 mM sodium sulfate. (e) Effects of culture medium pH on the growth of the three yeast strains grown in YNB liquid culture medium containing 0.1 mM sodium sulfate. (f) Uptake of 35S-labeled sulfate by Yp112-GmSULTR1;2b yeast cells. Sulfate uptake rates were calculated after transferring the cells to media containing different external sulfate concentrations at pH 6.0. Error bars represent the standard deviation. CP154-7A, CP154-7A mutant strain transformed with the empty vector (as a negative control); Yp112-AtSULTR1;2, CP154-7A transformed with Yp112-AtSULTR1;2 (as a positive control); Yp112-GmSULTR1;2b, CP154-7A transformed with Yp112-GmSULTR1;2b

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