Fig. 8
Biomass and content of sulfur-containing compounds in GmSULTR1;2b-overexpressing tobacco plants grown under -S conditions. (a) Dry weights of 2-month-old whole GmSULTR1;2b-overexpressing tobacco plants and whole control plants under -S (0 mM MgSO4) conditions. (b) Chlorophyll content of the leaves of GmSULTR1;2b-overexpressing tobacco plants and control plants grown under -S conditions for 2 weeks. (c) Soluble protein content in the functional leaves of 2-month-old GmSULTR1;2b-overexpressing tobacco plants and control plants grown under -S conditions. (d) Total thiol content in the functional leaves of 2-month-old GmSULTR1;2b-overexpressing tobacco plants and control plants grown under -S conditions. (e) Sulfate ion content in whole roots of 2-month-old GmSULTR1;2b-overexpressing tobacco plants and control plants grown under -S conditions. Three biological replicates were performed. Error bars represent the standard deviation. Asterisks (*) indicate significant differences at P < 0.05 (Student’s t-test), and double asterisks (**) indicate significant differences at P < 0.01 (Student’s t-test). CK, tobacco plant transformed with the pMDC83 empty vector as a control; S22, S26 and S29, tobacco plants transformed with GmSULTR1;2b, FW fresh weight